Picture of 12 Lipoxygenase Antibody

Picture of 12 Lipoxygenase Antibody

Picture of 12 Lipoxygenase Antibody

Picture of 12 Lipoxygenase Antibody

12 Lipoxygenase Antibody

Catalog Number: ASA-B0001

Lead time: 1 week

$299.00

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
arachidonate 12-lipoxygenase	Polyclonal	IgG	Rabbit	Human	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12(609-628aa HHKEKYFSGPKPKAVLNQFR), different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	ALOX12
Protein	Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase)
Uniprot ID	P18054
Function	Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
Tissue Specificity	Expressed in vascular smooth muscle cells.
Sub-cellular localization	Cytoplasm, cytosol. Membrane. Note: Membrane association is stimulated by EGF.
Sequence Similarities	Belongs to the lipoxygenase family.
Aliases	12 LOX antibody\|12(S) lipoxygenase antibody\|12S LOX antibody\|12S-lipoxygenase antibody\|12S-LOX antibody\|12S-type antibody\|ALOX12 antibody\|Alox12L antibody\|Alox15 antibody\|Arachidonate 12 lipoxygenase antibody\|Arachidonate 12 lipoxygenase, 12S type antibody\|Arachidonate 12 lipoxygenase, leukocyte type antibody\|Arachidonate 12-lipoxygenase antibody\|EC1.13.11.31 antibody\|LOG12 antibody\|LOX12_HUMAN antibody\|Platelet type lipoxygenase 12 antibody\|Platelet-type lipoxygenase 12 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolution's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information