ABCB11 Antibody

Catalog Number: ASA-B0016

Lead time: 1 week

$339.25

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
ATP-binding cassette, sub-family B (MDR/TAP), member 11	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11 (1175-1199aa KYGDNTKEIPMERVIAAAKQAQLHD), different from the related mouse and rat sequences by three amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	ABCB11
Protein	Bile salt export pump
Uniprot ID	O95342
Function	Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
Tissue Specificity	Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
Sub-cellular localization	Membrane; Multi-pass membrane protein.
Sequence Similarities	Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Aliases	ABC member 16, MDR/TAP subfamily antibody\|ABCB1 antibody\|Abcb11 antibody\| ABCBB_HUMAN antibody\|ATP binding cassette sub family B (MDR/TAP) member 11 antibody\|ATP binding cassette sub family B member 11 antibody\|ATP-binding cassette sub-family B member 11 antibody\|Bile salt export pump antibody\|BRIC2 antibody\|Bsep antibody\|PGY4 antibody\| progressive familial intrahepatic cholestasis 2 antibody\|Sister of P glycoprotein antibody\| Spgp antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- ABCB11 antibody, ASA-B0016, Western blotting
All lanes: Anti ABCB11 (ASA-B0016) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted band size: 146KD
Observed band size: 146KD

Anti- ABCB11 antibody, ASA-B0016, IHC(P)
Mouse Liver Tissue

Anti- ABCB11 antibody, ASA-B0016, IHC(P)
Rat Liver Tissue

ABCB11 Antibody

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification

Immunogen

Properties

Form

Size

Contents

Concentration

Storage

Additional Information Regarding the Antigen

Gene

Protein

Uniprot ID

Function

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

Application Details