Bovine Sphingosine Kinase 1/SPHK1 ELISA Kit

Catalog Number: AYQ-E10762
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect expiration date. from light. Do not use past
Gene ID 618605
Gene Symbol SPHK1
Synonym EC; SK1; sphingosine kinase 1; SPHK1; SPHKSK 1; SPK 1; SPK; SPK1
Species Bovine
Specificity This assay has high sensitivity and excellent specificity for detection of bovine Sphingosine Kinase 1/SPHK1. No significant cross-reactivity or interference between bovine Sphingosine Kinase 1/SPHK1 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 0.313 ng/ml - 20 ng/ml
Sensitivity 0.25 ng/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Bovine Sphingosine Kinase 1 SPHK1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. Sphingosine kinase 1 phosphorylates sphingosine to sphingosine-1-phosphate (S1P) SK1 is normally a cytosolic protein but is recruited to membranes rich in phosphatidate (PA), a product of Phospholipase D (PLD).