Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| cyclin D1 |
Polyclonal |
IgG |
Rabbit |
Human, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1(19-37aa DANLLNDRVLRAMLKAEET), different from the related mouse and rat sequences by two amino acids. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
CCND1 |
Protein
|
G1/S-specific cyclin-D1 |
Uniprot ID
|
P24385 |
Function
|
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
Tissue Specificity
|
|
Sub-cellular localization
|
Nucleus. |
Sequence Similarities
|
Belongs to the cyclin family. Cyclin D subfamily. |
Aliases
|
AI327039 antibody|B cell ccl/lymphoma 1 antibody|B cell CLL/lymphoma 1 antibody|B cell leukemia 1 antibody|B-cell leukemia 1 antibody|B cell lymphoma 1 protein antibody|B-cell lymphoma 1 protein antibody|BCL 1 antibody|BCL-1 antibody|BCL-1 oncogene antibody|BCL1 antibody|BCL1 oncogene antibody|CCND 1 antibody|CCND1 antibody|CCND1 protein antibody|CCND1/FSTL3 fusion gene, included antibody|CCND1/IGHG1 fusion gene antibody|CCND1/IGHG1 fusion gene, included antibody|CCND1/IGLC1 fusion gene, included antibody|CCND1/PTH fusion gene, included antibody|CCND1_HUMAN antibody|cD1 antibody|Cyl 1 antibody|D11S287E antibody|G1/S specific cyclin D1 antibody|G1/S-specific cyclin-D1 antibody|Parathyroid adenomatosis 1 antibody|PRAD1 antibody|PRAD1 oncogene antibody|U21B31 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Rat |
AssaySolution's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cyclin D1 antibody, ASA-B0532, All Western blotting
All lanes: Anti-CCND1(ASA-B0532) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Predicted band size: 33KD
Observed band size: 33KD
