Human Apolipoprotein A-I/ApoA1 ELISA Kit

Catalog Number: AYQ-E11176
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 335
Gene Symbol ApoA1
Synonym Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of human Apolipoprotein A-I/ApoA1. No significant cross-reactivity or interference between human Apolipoprotein A-I/ApoA1 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 3.13 ng/ml - 200 ng/ml
Sensitivity 2.5 ng/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. It has a specific role in lipid metabolism. The text in a recent report suggested that APOA1 mRNA is regulated by endogenously expressed antisense RNA. Apolipoprotein A1 is the major protein component of HDL particles in plasma. Chylomicrons secreted from the intestinal enterocyte also contain apo A1, but it is quickly transferred to HDL in the bloodstream. The protein, as a component of HDL particles, enables efflux of fat molecules by accepting fats from within cells (including macrophages within the walls of arteries which have become overloaded with ingested fats from oxidized LDL particles) for transport (in the water outside cells) elsewhere, including back to LDL particles or to the liver for excretion.