Catalog Number: AYQ-E10818
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 2352
Gene Symbol FOLR3
Synonym folate receptor 3 (gamma); Folate receptor 3; folate receptor gamma; FOLR3; FR-G; FR-gamma; gamma-hFR
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human FOLR3 . No significant cross-reactivity or interference between Human FOLR3 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 23.4--1500 pg/ml
Sensitivity 18.75 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human FOLR3 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Folate receptor 3 (FOLR3), also known as folate receptor gamma, is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence. A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein. Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa. FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas. FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas. Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates.