Human Hyaluronidase 1/HYAL1 ELISA Kit

Catalog Number: AYQ-E11273
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 3373
Gene Symbol HYAL1
Synonym EC; FUS2; HYAL1; HYAL-1; Hyaluronidase 1; Hyaluronoglucosaminidase 1; hyaluronoglucosaminidase-1; LUCA1; luCa-1; LUCA1hyaluronidase-1; Lung carcinoma protein 1; MGC45987; NAT6; plasma hyaluronidase; tumor suppressor LUCA-1
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of human Hyaluronidase 1/HYAL1. No significant cross-reactivity or interference between human Hyaluronidase 1/HYAL1 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 12.5 pg/ml - 800 pg/ml
Sensitivity 10 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Hyaluronidase 1/HYAL1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Hyaluronidase-1 is an enzyme that in humans is encoded by the HYAL1 gene. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.