Human IL-36Ra/IL-1F5 ELISA Kit

Catalog Number: AYQ-E10984
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 26525
Gene Symbol IL-36RA
Synonym FIL1 delta; IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; Interleukin 36 Receptor Antagonist; Interleukin-36 Receptor Antagonist
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human IL-36Ra/IL-1F5 . No significant cross-reactivity or interference between Human IL-36Ra/IL-1F5 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 93.8--6000 pg/ml
Sensitivity 75 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human IL-36Ra/IL-1F5 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background In humans, there are 4 distinct interleukin-36 genes. They are, three receptor agonists: IL36A, IL36B, and IL36G, and one receptor antagonist IL36RA that bind the IL-36 receptor (IL1RL2/IL-1Rrp2/IL-36 receptor) with varying affinities. The agonists are known to activate NF-κB and mitogen-activated protein kinases to induce various proinflammatory mediators. It has also been found to activate T cell proliferation and release of IL-2. Due to their predominant expression in epithelial tissues, IL-36 cytokines are believed to play a significant role in the pathogenesis of skin diseases, especially that of psoriasis.