Human Pepsinogen A4 ELISA Kit

Catalog Number: AYQ-E11105
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 643847
Gene Symbol PGA4
Synonym Pepsin A-4; Pepsinogen A4; PGA4
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of human Pepsinogen A4. No significant cross-reactivity or interference between human Pepsinogen A4 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 0.313 ng/ml - 20 ng/ml
Sensitivity 0.25 ng/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Pepsinogen A4 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Pepsin A (EC, pepsin, lactated pepsin, pepsin fortior, fundus-pepsin, elixir lactate of pepsin, P I, lactated pepsin elixir, P II, pepsin R, pepsin D) is an enzyme. This enzyme catalyses the following chemical reaction. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves Phe1-Val, Gln4-His, Glu13-Ala, Ala14-Leu, Leu15-Tyr, Tyr16-Leu, Gly23-Phe, Phe24-Phe and Phe25-Tyr bonds in the B chain of insulin. The enzyme is a predominant endopeptidase in the gastric juice of vertebrates.