Human Procalcitonin ELISA Kit

Catalog Number: AYQ-E11280
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 796
Gene Symbol Procalcitonin
Synonym ATP2B2plasma membrane calcium ATPase; ATPase, Ca++ transporting, plasma membrane 4; CALC1; CALCA; Calcitonin 1; CGRP; CT; DKFZp686G08106; DKFZp686M088; EC 3.6.3; EC; Katacalcin; Matrix-remodeling-associated protein 1; matrix-remodelling associated 1; MXRA1; PCT; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; plasma membrane calcium pump; plasma membrane calcium-transporting ATPase 4; PMCA4b; PMCA4PMCA4x; Preprocalcitonin; Procalcitonin; sarcolemmal calcium pump
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of human Procalcitonin. No significant cross-reactivity or interference between human Procalcitonin and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 31.3 pg/ml - 2000 pg/ml
Sensitivity 25 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Procalcitonin ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It was first identified by Leonard J. Deftos and Bernard A. Roos in the 1970s. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a proinflammatory stimulus, especially of bacterial origin. It is therefore often classed as an acute phase reactant. In this case, it is produced mainly by the cells of the lung and the intestine. It does not rise significantly with viral or non-infectious inflammations. With the derangements that a severe infection with an associated systemic response brings, the blood levels of procalcitonin may rise to 100 µg/L. In serum, procalcitonin has a half-life of 25 to 30 hours. Remarkably the high procalcitonin levels produced during infections are not followed by a parallel increase in calcitonin or a decrease in serum calcium levels.