Human SPARC-like 1/SPARCL1 ELISA Kit

Catalog Number: AYQ-E11171
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 8404
Gene Symbol SPARCL1
Synonym Hevin; High endothelial venule protein; MAST 9; MAST9; PIG33; proliferation-inducing protein 33; SC1; SPARC like 1; SPARCL1; SPARC-like 1 (hevin); SPARC-like 1 (mast9, hevin); SPARC-like 1; SPARC-like protein 1
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of human CD31/PECAM-1. No significant cross-reactivity or interference between human CD31/PECAM-1 and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 46.9 pg/ml - 3000 pg/ml
Sensitivity 37.5 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human CD31/PECAM-1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background SPARC-like protein 1 (SPARCL1 or SC1), also known as hevin (short for high endothelial venule protein), is a secreted protein with high structural similarity to SPARC. It interacts with the extracellular matrix to create intermediate states of cell adhesion. Due to its dynamic extracellular roles, being implicated in cancer metastasis and inflammation, it is considered a matricellular protein. In humans hevin is encoded by the SPARCL1 gene.