Human Thrombin-antithrombin(TAT) ELISA Kit

Catalog Number: AYQ-E11321
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 2147
Gene Symbol TAT
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human Thrombin-antithrombin(TAT) . No significant cross-reactivity or interference between Human Thrombin-antithrombin(TAT) and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 100 - 6250 pg/ml
Sensitivity 80 pg/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Thrombin-antithrombin(TAT) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Thrombin (Factor IIa) is an enzyme, a serine protease, which participates in blood clotting. It is formed when prothrombin (Factor II) is proteolytically cleaved to form thrombin, which is glycoprotein formed by two peptides chains of 36 and 259 amino acids. Thrombin converts soluble fibrinogen into insoluble strands of fibrin and is then neutralized by antithrombin in order to for a thrombin-antithrombin (TAT) complex.