12 Lipoxygenase Antibody

Catalog Number: ASA-B0001
Lead time: 1 week


Long Name

Antibody Type

Antibody Isotype


Species Reactivity

Validated Applications


arachidonate 12-lipoxygenase Polyclonal IgG Rabbit Human WB Immunogen affinity purified.


A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12(609-628aa HHKEKYFSGPKPKAVLNQFR), different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.





100 µg/vial


Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.


Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).


At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen




Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase)

Uniprot ID



Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.

Tissue Specificity

Expressed in vascular smooth muscle cells.

Sub-cellular localization

Cytoplasm, cytosol. Membrane. Note: Membrane association is stimulated by EGF.

Sequence Similarities

Belongs to the lipoxygenase family.


12 LOX antibody|12(S) lipoxygenase antibody|12S LOX antibody|12S-lipoxygenase antibody|12S-LOX antibody|12S-type antibody|ALOX12 antibody|Alox12L antibody|Alox15 antibody|Arachidonate 12 lipoxygenase antibody|Arachidonate 12 lipoxygenase, 12S type antibody|Arachidonate 12 lipoxygenase, leukocyte type antibody|Arachidonate 12-lipoxygenase antibody|EC1.13.11.31 antibody|LOG12 antibody|LOX12_HUMAN antibody|Platelet type lipoxygenase 12 antibody|Platelet-type lipoxygenase 12 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information