14-3-3 sigma Antibody

Catalog Number: ASA-B0002
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

stratifin Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

SFN

Protein

14-3-3 protein sigma

Uniprot ID

P31947

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.

Tissue Specificity

Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Sub-cellular localization

Cytoplasm. Nucleus . Secreted. Note: May be secreted by a non-classical secretory pathway.

Sequence Similarities

 

Aliases

14 3 3 protein sigma antibody|14-3-3 protein sigma antibody|1433S_HUMAN antibody|Epithelial cell marker protein 1 antibody|Er antibody|HME 1 antibody|HME1 antibody|MGC143283 antibody|Mkrn3 antibody|Mme1 antibody|OTTHUMP00000004242 antibody|RP23 137L22.11 antibody|SFN antibody|SFN protein antibody|Stratifin antibody|YWHAS antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human Mouse, Rat AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human Mouse, Rat AssaySolution's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, Rat AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-14-3-3 sigma antibody, ASA-B0002, Western blotting
All lanes: Anti 14-3-3 sigma (ASA-B0002) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SE620 Whole Cell Lysate at 40ug
Predicted band size: 28KD
Observed band size: 28KD
 
Anti-14-3-3 sigma antibody, ASA-B0002, IHC (P) 
Human Tonsil Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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