5 Lipoxygenase Antibody

Catalog Number: ASA-B0003
Lead time: 1 week


Long Name

Antibody Type

Antibody Isotype


Species Reactivity

Validated Applications


arachidonate 5-lipoxygenase Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.


A synthetic peptide corresponding to a sequence at the C-terminus of human 5 Lipoxygenase(650-667aa AERNKKKQLPYYYLSPDR), different from the related rat and mouse sequences by two amino acids.





100 µg/vial


Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.


Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).


At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen




Arachidonate 5-lipoxygenase(5-LO/5-lipoxygenase)

Uniprot ID



Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Tissue Specificity


Sub-cellular localization

Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Sequence Similarities

Belongs to the lipoxygenase family.


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Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-5 Lipoxygenase antibody, ASA-B0003, Western blotting
All lanes: Anti 5 Lipoxygenase (ASA-B0003) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted band size: 78KD
Observed band size: 78KD
Anti-5 Lipoxygenase antibody, ASA-B0003, IHC(P)
Human Lung Cancer Tissue
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.