5 Lipoxygenase Antibody

Catalog Number: ASA-B0003
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

arachidonate 5-lipoxygenase Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 5 Lipoxygenase(650-667aa AERNKKKQLPYYYLSPDR), different from the related rat and mouse sequences by two amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ALOX5

Protein

Arachidonate 5-lipoxygenase(5-LO/5-lipoxygenase)

Uniprot ID

P09917

Function

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Tissue Specificity

 

Sub-cellular localization

Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Sequence Similarities

Belongs to the lipoxygenase family.

Aliases

5 LO antibody|5 LOX antibody|5-lipoxygenase antibody|5-LO antibody|5-LOX antibody|5LOX antibody|5LPG antibody|ALOX 5 antibody|Alox5 antibody|Arachidonate 5 lipoxygenase antibody|Arachidonate 5-lipoxygenase antibody|arachidonic 5-lipoxygenase alpha-10 isoform antibody|arachidonic 5-lipoxygenase delta-10-13 isoform antibody|arachidonic 5-lipoxygenase delta-13 isoform antibody|arachidonic 5-lipoxygenase delta-p10 isoform antibody|Arachidonic acid 5 lipoxygenase antibody|Leukotriene A4 synthase antibody|LOG 5 antibody|LOG5 antibody|LOX5_HUMAN antibody|MGC163204 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-5 Lipoxygenase antibody, ASA-B0003, Western blotting
All lanes: Anti 5 Lipoxygenase (ASA-B0003) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted band size: 78KD
Observed band size: 78KD
 
Anti-5 Lipoxygenase antibody, ASA-B0003, IHC(P)
Human Lung Cancer Tissue
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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