53BP1 Antibody

Catalog Number: ASA-B0004
Lead time: 1 week


Long Name

Antibody Type

Antibody Isotype


Species Reactivity

Validated Applications


tumor protein p53 binding protein 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.


A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.





100 µg/vial


Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.


Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).


At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen




Tumor suppressor p53-binding protein 1

Uniprot ID



Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53- mediated transcriptional activation.

Tissue Specificity


Sub-cellular localization

Nucleus. Chromosome, centromere, kinetochore. Note: Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed.

Sequence Similarities

Contains 2 BRCT domains.


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Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse Rat AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat  AssaySolution's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, Rat AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-53BP1 antibody, ASA-B0004, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: U20S Cell Lysate
Lane 3: HEPA Cell Lysate
Anti-53BP1 antibody, ASA-B0004, IHC(P)
Human Intestinal Cancer Tissue
Anti-53BP1 antibody, ASA-B0004, IHC(P)
Rat Brain Tissue
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.