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53BP1 Antibody

Catalog Number: ASA-B0004
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

tumor protein p53 binding protein 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

TP53BP1

Protein

Tumor suppressor p53-binding protein 1

Uniprot ID

Q12888

Function

Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53- mediated transcriptional activation.

Tissue Specificity

 

Sub-cellular localization

Nucleus. Chromosome, centromere, kinetochore. Note: Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed.

Sequence Similarities

Contains 2 BRCT domains.

Aliases

53 BP1 antibody|53BP1 antibody|FLJ41424 antibody|MGC138366 antibody|p202 antibody|p53 binding protein 1 antibody|p53 BP1 antibody|p53-binding protein 1 antibody|p53BP1 antibody|TP53 BP1 antibody|TP53B_HUMAN antibody|TP53BP1 antibody|TRP53 BP1 antibody|Tumor protein 53 binding protein 1 antibody|Tumor protein p53 binding protein 1 antibody|Tumor suppressor p53 binding protein 1 antibody|Tumor suppressor p53-binding protein 1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse Rat AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat  AssaySolution's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, Rat AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-53BP1 antibody, ASA-B0004, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: U20S Cell Lysate
Lane 3: HEPA Cell Lysate
 
Anti-53BP1 antibody, ASA-B0004, IHC(P)
Human Intestinal Cancer Tissue
 
Anti-53BP1 antibody, ASA-B0004, IHC(P)
Rat Brain Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.