5HT2B Receptor Antibody

Catalog Number: ASA-B0008
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled Polyclonal IgG Rabbit Human, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2B Receptor (446-478aa MRLRSSTIQSSSIILLDTLLLTENEGDKTEEQ V), different from the related mouse sequence by six amino acids, and from the related rat sequence by nine amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

HTR2B

Protein

5-hydroxytryptamine receptor 2B

Uniprot ID

P41595

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down- stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5- hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine.

Tissue Specificity

Ubiquitous. Detected in liver, kidney, heart, pulmonary artery, and intestine. Detected at lower levels in blood, placenta and brain, especially in cerebellum, occipital cortex and frontal cortex.

Sub-cellular localization

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome .

Sequence Similarities

Belongs to the G-protein coupled receptor 1 family.

Aliases

5 HT 2B antibody|5 HT 2B receptor antibody|5 HT(2B) antibody|5 HT2B antibody|5 hydroxytryptamine (serotonin) receptor 2B antibody|5 hydroxytryptamine (serotonin) receptor 2B G protein coupled antibody|5 hydroxytryptamine 2B receptor antibody|5 hydroxytryptamine receptor 2B antibody|5-HT-2B antibody|5-HT2B antibody|5-hydroxytryptamine receptor 2B antibody|5HT(2B) antibody|5HT2B_HUMAN antibody| 5HT2F antibody|Htr 2b antibody|Htr2b antibody|NP75 protein antibody|SEROTONIN 5HT2B RECEPTOR antibody|Serotonin receptor 2B antibody| Stomach fundus serotonin receptor antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti- 5HT2B
Anti- 5HT2B Receptor antibody, ASA-B0008, Western blotting
All lanes: Anti 5HT2B Receptor (ASA-B0008) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted band size: 54KD
Observed band size: 54KD
 
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Newsletter