ABCA4 Antibody

Catalog Number: ASA-B0014
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ATP-binding cassette, sub-family A(ABC1), member 4 Polyclonal IgG Rabbit Mouse, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse ABCA4(1892-1903aa TLLIQHHFFLTR), identical to the related rat sequence.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ABCA4

Protein

Retinal-specific ATP-binding cassette transporter

Uniprot ID

O35600

Function

In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans- retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery (By similarity).

Tissue Specificity

Retinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells.

Sub-cellular localization

Membrane ; Multi-pass membrane protein . Note: Localized to outer segment disk edges of rods and cones, with around one million copies/photoreceptor.

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCA family.

Aliases

ABC 10 antibody|ABC A4 antibody|ABC10 antibody|ABCA 4 antibody|abcA4 antibody|ABCA4_HUMAN antibody|ABCR antibody|ARMD 2 antibody|ARMD2 antibody|ATP binding cassette 10 antibody|ATP binding cassette sub family A member 4 antibody|ATP binding cassette sub family A member4 antibody|ATP binding cassette transporter antibody|ATP binding cassette transporter retinal specific antibody|ATP binding cassette, sub family A(ABC1), member 4 antibody|ATP binding cassette, sub family A(ABC1), member4 antibody|ATP binding cassette10 antibody|ATP binding transporter, retina specific antibody|ATP-binding cassette sub-family A member 4 antibody|CORD 3 antibody|CORD3 antibody|DKFZp781N1972 antibody|FFM antibody|FLJ17534 antibody|Photoreceptor rim protein antibody|Retina specific ABC transporter antibody|Retinal specific ATP binding cassette transporter antibody|Retinal-specific ATP-binding cassette transporter antibody|RIM ABC transporter antibody|RIM protein antibody|RmP antibody|RP 19 antibody|RP19 antibody|Stargardt disease protein antibody|STGD antibody|STGD1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse Rat AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-ABCA4 antibody, ASA-B0014, Western blotting
All lanes: Anti ABCA4 (ASA-B0014) at 0.5ug/ml
WB: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 256KD
Observed bind size: 256KD
 
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Newsletter