ABCB11 Antibody

Catalog Number: ASA-B0016
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ATP-binding cassette, sub-family B (MDR/TAP), member 11 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11 (1175-1199aa KYGDNTKEIPMERVIAAAKQAQLHD), different from the related mouse and rat sequences by three amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ABCB11

Protein

Bile salt export pump

Uniprot ID

O95342

Function

Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.

Tissue Specificity

Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.

Sub-cellular localization

Membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Aliases

ABC member 16, MDR/TAP subfamily antibody|ABCB1 antibody|Abcb11 antibody| ABCBB_HUMAN antibody|ATP binding cassette sub family B (MDR/TAP) member 11 antibody|ATP binding cassette sub family B member 11 antibody|ATP-binding cassette sub-family B member 11 antibody|Bile salt export pump antibody|BRIC2 antibody|Bsep antibody|PGY4 antibody| progressive familial intrahepatic cholestasis 2 antibody|Sister of P glycoprotein antibody| Spgp antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, Rat AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- ABCB11
Anti- ABCB11 antibody, ASA-B0016, Western blotting
All lanes: Anti ABCB11 (ASA-B0016) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted band size: 146KD
Observed band size: 146KD
 
Anti- ABCB11mouse
Anti- ABCB11 antibody, ASA-B0016, IHC(P)
Mouse Liver Tissue
 
Anti- ABCB11rat
Anti- ABCB11 antibody, ASA-B0016, IHC(P)
Rat Liver Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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