ABCB4 Antibody

Catalog Number: ASA-B0018
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ATP-binding cassette, sub-family B (MDR/TAP), member 4 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ABCB4

Protein

Multidrug resistance protein 3

Uniprot ID

P21439

Function

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

Tissue Specificity

 

Sub-cellular localization

Cell membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Aliases

ABC 21 antibody|ABC B4 antibody|ABC21 antibody|ABCB 4 antibody|Abcb4 antibody|ABCB4 protein antibody|ATP binding cassette sub family B MDR/TAP member 4 antibody|ATP binding cassette sub family B member 4 antibody|ATP-binding cassette sub-family B member 4 antibody|MDR 3 antibody|MDR2 antibody|MDR2/3 antibody|MDR3 antibody|MDR3 P glycoprotein antibody|MDR3 P gp antibody|MDR3_HUMAN antibody|Multidrug resistance protein 3 antibody|Multiple drug resistance 3 antibody|P glycoprotein 3 antibody|P-glycoprotein 3 antibody|PFIC 3 antibody|PFIC3 antibody|PGY 3 antibody|PGY3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- ABCB4
Anti- ABCB4 antibody, ASA-B0018, Western blotting
All lanes: Anti ABCB4 (ASA-B0018) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted band size: 142KD
Observed band size: 142KD
 
Anti- ABCB4mouse
Anti- ABCB4 antibody, ASA-B0018, IHC(P)
Mouse Intestine Tissue
 
Anti- ABCB4rat
Anti- ABCB4 antibody, ASA-B0018, IHC(P)
Rat Liver Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Newsletter