ABCG5 Antibody

Catalog Number: ASA-B0022
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ATP-binding cassette, sub-family G(WHITE), member 5 Polyclonal IgG Rabbit Human WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5(116-131aa NGRALRREQFQDCFSY).

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ABCG5

Protein

ATP-binding cassette sub-family G member 5

Uniprot ID

Q9H222

Function

Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

Tissue Specificity

Strongly expressed in the liver, lower levels in the small intestine and colon.

Sub-cellular localization

Membrane ; Multi-pass membrane protein .

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Aliases

ABCG5 antibody|ABCG5_HUMAN antibody|ATP binding cassette sub family G(WHITE) member 5(sterolin 1) antibody|ATP binding cassette sub family G member 5 antibody|ATP-binding cassette sub-family G member 5 antibody|Sterolin 1 antibody|Sterolin-1 antibody|STSL antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-ABCG5 antibody, ASA-B0022, Western blotting
All lanes: Anti ABCG5 (ASA-B0022) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted band size: 73KD
Observed band size: 73KD
 
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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