Actin Antibody

Catalog Number: ASA-B0030
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

actin, gamma 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin(365-375aa SGPSIVHRKCF), identical to the related mouse and rat sequences.

Properties

Form

Liquid

Size

100 µg/vial

Contents

Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ACTG1

Protein

Actin, cytoplasmic 2

Uniprot ID

P63261

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Tissue Specificity

 

Sub-cellular localization

Cytoplasm, cytoskeleton.

Sequence Similarities

Belongs to the actin family.

Aliases

Gamma-actin

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat Mouse AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat Mouse AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Actin antibody, ASA-B0030, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: MM231 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: SW620 Cell Lysate
 
Anti-Actin antibody, ASA-B0030, IHC(P)
Rat Cardiac Muscle Tissue
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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