Actin Antibody

Catalog Number: ASA-B0031
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

actin, alpha 1, skeletal muscl Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ACTA1

Protein

Actin, alpha skeletal muscle

Uniprot ID

P68133

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Tissue Specificity

 

Sub-cellular localization

Cytoplasm, cytoskeleton.

Sequence Similarities

 

Aliases

ACTA antibody|ACTA1 antibody|Actin alpha skeletal muscle antibody|Actin antibody|actin, alpha 1, skeletal muscle 1 antibody|actin, alpha 1, skeletal muscle antibody|Actin, alpha skeletal muscle antibody|actina antibody|actine antibody| ACTS_HUMAN antibody|aktin antibody|Alpha Actin 1 antibody|Alpha skeletal muscle Actin antibody|alpha skeletal muscle antibody|alpha-actin antibody|Alpha-actin-1 antibody|ASMA antibody|CFTD antibody| CFTD1antibody|CFTDM antibody| MPFD antibody|NEM1 antibody|NEM2 antibody|NEM3 antibody| nemaline myopathy type 3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, Rat AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- ACTIN antibody, ASA-B0031, Western blotting
All lanes: Anti ACTIN (ASA-B0031) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted band size: 42KD
Observed band size: 42KD
 
Anti- ACTIN antibody, ASA-B0031, IHC(P)
Mouse Intestine Tissue
 
Anti- ACTIN antibody, ASA-B0031, IHC(P)
Rat Spleen Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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