ADAM17 Antibody

Catalog Number: ASA-B0033
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ADAM metallopeptidase domain 17 Polyclonal IgG Rabbit Human, Mouse, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17(806-824aa AASFKLQRQNRVDSKETEC), different from the related rat and mouse sequences by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ADAM17

Protein

Disintegrin and metalloproteinase domain-containing protein 17(ADAM 17)

Uniprot ID

P78536

Function

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.

Tissue Specificity

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

Sub-cellular localization

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 disintegrin domain.

Aliases

A disintegrin and metalloproteinase domain 17(tumor necrosis factor, alpha, converting enzyme) antibody|A disintegrin and metalloproteinase domain 17 antibody|ADA17_HUMAN antibody|ADAM 17 antibody|ADAM metallopeptidase domain 17 antibody|ADAM17 antibody|ADAM17 protein antibody|CD 156b antibody|CD156b antibody|CD156b antigen antibody|CSVP antibody|Disintegrin and metalloproteinase domain-containing protein 17 antibody|MGC71942 antibody|Snake venom like protease antibody|Snake venom-like protease antibody|TACE antibody|TNF alpha convertase antibody|TNF alpha converting enzyme antibody|TNF-alpha convertase antibody|TNF-alpha-converting enzyme antibody|Tumor Necrosis Factor Alpha Converting Enzyme antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human Mouse, Rat AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-ADAM17 antibody, ASA-B0033, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: 293T Cell Lysate
 
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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