ADAMTS5 Antibody

Catalog Number: ASA-B0039
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ADAM metallopeptidase with thrombospondin type 1 motif, 5 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS5(772-787aa TAYLALKKKNGEYLIN), different from the related mouse and rat sequence by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ADAMTS5

Protein

A disintegrin and metalloproteinase with thrombospondin motifs 5

Uniprot ID

Q9UNA0

Function

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Tissue Specificity

Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.

Sub-cellular localization

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Contains 1 disintegrin domain.

Aliases

A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody|A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody|A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 5 antibody|A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif, 5(aggrecanase 2) antibody|A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody|ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody|ADAM TS 11 antibody|ADAM TS 5 antibody|ADAM TS5 antibody|ADAM-TS 11 antibody|ADAM-TS 5 antibody|ADAM-TS5 antibody|ADAMTS 11 antibody|ADAMTS 5 antibody|ADAMTS-11 antibody|ADAMTS-5 antibody|ADAMTS11 antibody|Adamts5 antibody|ADMP 2 antibody|ADMP-2 antibody|ADMP2 antibody|Aggrecanase 2 antibody|Aggrecanase-2 antibody|ATS5_HUMAN antibody|FLJ36738 antibody|Implantin antibody|ThromboSpondin motif-5 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat Mouse AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Rat Human, Mouse AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-ADAMTS5 antibody, ASA-B0039, IHC(P)
Rat Brain Tissue
 
Anti-ADAMTS5 antibody, ASA-B0039, Western blotting
All lanes: Anti ADAMTS5 (ASA-B0039) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 102KD
Observed bind size: 102KD
 
Anti-ADAMTS5 antibody, ASA-B0039, IHC(P)
Rat Brain Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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