ADRA1A Antibody

Catalog Number: ASA-B0044
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

adrenoceptor alpha 1A Polyclonal IgG Rabbit Human WB Immunogen affinity purified.

Immunogen

E.coli-derived human ADRA1A recombinant protein (Position: R235-V466). Human ADRA1A shares 89% and 90% amino acid (aa) sequences identity with mouse and rat ADRA1A, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ADRA1A

Protein

Alpha-1A adrenergic receptor

Uniprot ID

P35348

Function

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

Tissue Specificity

Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

Sub-cellular localization

Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.

Sequence Similarities

 

Aliases

ADA1D_HUMAN antibody|Adra1 antibody|Adra1a antibody|Adra1d antibody|ADRA1R antibody|Adrd1 antibody|Adrenergic alpha1A receptor antibody|Adrenergic alpha1D receptor antibody|Adrenergic receptor alpha 1d antibody|Adrenergic receptor delta1 antibody|Adrenoceptor alpha 1D antibody|Alpha 1D adrenoceptor antibody|Alpha 1D adrenoreceptor antibody|Alpha adrenergic receptor 1a antibody|Alpha-1A adrenergic receptor antibody|Alpha-1D adrenergic receptor antibody|Alpha-1D adrenoceptor antibody|Alpha-1D adrenoreceptor antibody|Alpha-adrenergic receptor 1a antibody|ALPHA1 antibody|Alpha1A adrenergic receptor antibody|Alpha1D adrenergic receptor antibody|Alpha1DAR antibody|DAR antibody|dJ779E11.2 antibody|Gpcr8 antibody|RA42 antibody|Spr8 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit


AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-ADRA1A
Anti-ADRA1A antibody, ASA-B0044, western blotting
All lanes: Anti-ADRA1A(ASA-B0044) at 0.5ug/ml
WB: Recombinant Human ADRA1A Protein 0.5ng
Predicted bind size: 51KD
Observed bind size: 51KD
 
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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