AFP Antibody

Catalog Number: ASA-B0048
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

alpha-fetoprotein Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

AFP

Protein

Alpha-fetoprotein

Uniprot ID

P02771

Function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Tissue Specificity

Plasma. Synthesized by the fetal liver and yolk sac.

Sub-cellular localization

Secreted.

Sequence Similarities

Belongs to the ALB/AFP/VDB family.

Aliases

AFP antibody|Alpha fetoglobulin antibody|Alpha fetoprotein antibody|Alpha fetoproteinprecursor antibody|Alpha-1-fetoprotein antibody|Alpha-fetoglobulin antibody|Alpha-fetoprotein antibody|alpha-fetoprotein, Hereditary persistence of, included antibody|FETA antibody|FETA_HUMAN antibody|Hereditary persistence of alpha fetoprotein antibody|HPAFP antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, Rat AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

 
Anti-AFP
 
Anti-AFP antibody, ASA-B0048 Western blotting
All lanes: Anti AFP (ASA-B0048) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted band size: 69KD
Observed band size: 69KD
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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