AGFG1 Antibody

Catalog Number: ASA-B0049
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

ArfGAP with FG repeats 1 Polyclonal IgG Rabbit Human, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1(5-28aa AKRKQEEKHLKMLRDMTGLPHNRK), identical to the related mouse and rat sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

AGFG1

Protein

Arf-GAP domain and FG repeat-containing protein 1

Uniprot ID

P52594

Function

Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.

Tissue Specificity

Ubiquitously expressed.

Sub-cellular localization

Nucleus. Cytoplasmic vesicle.

Sequence Similarities

Contains 1 Arf-GAP domain.

Aliases

AGFG1 antibody|AGFG1_HUMAN antibody|Arf-GAP domain and FG repeats-containing protein 1 antibody|ArfGAP with FG repeats 1 antibody|AU045498 antibody|C130049H11Rik antibody|C85612 antibody|D730048C23Rik antibody| DKFZp686I15205 antibody|HIV 1 Rev binding protein antibody| HIV-1 Rev-binding protein antibody|HRB antibody|MGC116375 antibody|MGC116938 antibody| MGC116940 antibody|Nucleoporin like protein RIP antibody|Nucleoporin-like protein RIP antibody|RAB antibody|Rev interacting protein antibody|Rev-interacting protein antibody| Rev/Rex activation domain-binding protein antibody|RIP antibody|

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti- AGFG1 antibody, ASA-B0049, Western blotting
All lanes: Anti AGFG1 (ASA-B0049) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted band size: 58KD
Observed band size: 58KD
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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