AHR Antibody

Catalog Number: ASA-B0052
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

aryl hydrocarbon receptor Polyclonal IgG Rabbit Mouse, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse AHR (525-552aa DNKNNDLYNIMRNLGIDFEDIRSMQNEE), different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

AHR

Protein

Aryl hydrocarbon receptor

Uniprot ID

P30561

Function

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.

Tissue Specificity

Expressed in all tissues tested including brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen and thymus.

Sub-cellular localization

Cytoplasm. Nucleus. Note: Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

Sequence Similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Aliases

Ah receptor antibody|AHR antibody|AHR_HUMAN antibody|Aromatic hydrocarbon receptor antibody|Aryl hydrocarbon receptor antibody|Aryl hydrocarbon receptor precursor antibody| bHLHe76 antibody|Class E basic helix loop helix protein 76 antibody|Class E basic helix-loop-helix protein 76 antibody|HGNC:348 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse, Rat AssaySolution's ECL kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti- AHR
Anti- AHR antibody, ASA-B0052, Western blotting
All lanes: Anti AHR (ASA-B0052) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted band size: 38KD
Observed band size: 38KD
 
 
 
 
 
 
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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