AntiCD8 alpha antibody (polyclonal)
Human CD8 alpha recombinant protein (amino acids: 22-187).
No cross reactivity with other proteins
Immunogen affinity purified.
Lyophilized powder containg 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
*carrier free antibody available upon request.
Reconstitutiing with 1 ml of distilled water will yield a concentration of 100 µg/mL.
Western blot 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section) 0.5-1μg/ml
Note: The optimal working dilution should be determined by the end user.
Store at -20°C for one year. After reconstitution, sotre at 4°C for one month. store at -20˚C for a longer time. Aliquot to avoid repeated freezing and thawing.
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a
Anti- CD 8 alpha antibody, A0214B, IHC(P)
Human Tonsil Tissue
Anti- CD 8 alpha antibody, A0214B, Western blottingAll lanes: Anti CD 8 alpha (A0214B) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: RAJI Whole Cell Lysate at 40ugLane 3: HL-60 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugPredicted band size: 26KDObserved band size: 55KD