ARL15 Human

ARL15 is a member of the ARF family which are essential in eukaryotic vesicular trafficking pathways and have a vital part in the activation of phospholipase D. ARL15 variants influence levels of Acrp30, an adipocyte-derived protein that is extremely genetic and inversely related to the prospect of type 2 diabetes mellitus and coronary heart disease.
Catalog Number: AS-P00302
Lead time: 3-4 business days
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$0.00
ARL15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-204) and having a molecular mass of 25.0 kDa.ARL15 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Products specifications
Expression host E.coli.
Purity Greater than 85% as determined by SDS-PAGE.
Formulation The ARL15 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
Synonyms ADP-ribosylation factor-like 15, ADP-ribosylation factor related protein 2, ARFRP2, ARF-related protein 2, FLJ20051.
Reagent Appearance Sterile Filtered colorless solution.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MSDLRITEAF LYMDYLCFRA LCCKGPPPAR PEYDLVCIGL TGSGKTSLLS KLCSESPDNV VSTTGFSIKA VPFQNAILNV KELGGADNIR KYWSRYYQGS QGVIFVLDSA SSEDDLEAAR NELHSALQHP QLCTLPFLIL ANHQDKPAAR SVQEIKKYFE LEPLARGKRW ILQPCSLDDM DALKDSFSQL INLLEEKDHE AVRM
Application 1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin.2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin. 3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.