Beclin 1 Antibody

Catalog Number: ASA-B0216
Lead time: 1 week
$295.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

beclin 1, autophagy related Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

BECN1

Protein

Beclin-1

Uniprot ID

Q14457

Function

Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.

Tissue Specificity

Ubiquitous.

Sub-cellular localization

Cytoplasm.

Sequence Similarities

 

Aliases

APG6 antibody|ATG 6 antibody|ATG6 antibody|ATG6 autophagy related 6 homolog antibody|Bcl-2-interacting protein beclin antibody|Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody|Beclin 1 autophagy related antibody|Beclin-1 antibody|Beclin1 antibody|BECN 1 antibody|BECN1 antibody|BECN1_HUMAN antibody|Coiled coil myosin like BCL2 interacting protein antibody|Coiled-coil myosin-like BCL2-interacting protein antibody|GT197 antibody|Protein GT197 antibody|VPS 30 antibody|VPS30 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Mouse, Rat Human AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Beclin 1 antibody, ASA-B0216--1.jpg
IHC(P): Mouse Intestine Tissue
 
Anti-Beclin 1 antibody, ASA-B0216--2.jpg
IHC(P): Mouse Spleen Tissue
 
Anti-Beclin 1 antibody, ASA-B0216--3.jpg
IHC(P): Rat Intestine Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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