Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Gene ID
|
487475 |
Gene Symbol
|
DAN |
Synonym
|
DAN domain family member 1; DAN; DAND1; MGC8972; NBL1; neuroblastoma suppressor of tumorigenicity 1; neuroblastoma, suppression of tumorigenicity 1; Protein N03; suppression of tumorigenicity 1; Zinc finger protein DAN |
Species
|
canine |
Specificity
|
This assay has high sensitivity and excellent specificity for detection Canine DAN. No significant cross-reactivity or Canine DAN and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
125--8000 pg/ml |
Sensitivity
|
100 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Canine DAN ELISA Kit (Colorimetric)These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chicken Caronte, and C. elegans CeCan1. |