Canine eNOS ELISA Kit

Catalog Number: AYQ-E10699

Lead time: 3-4 business days

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Storage	Store the unopened product at 2 - 8° C. Protect expiration date. from light. Do not use past
Gene ID	403784
Gene Symbol	eNOS
Synonym	Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII
Species	Canine
Specificity	This assay has high sensitivity and excellent specificity for detection of canine eNOS. No significant cross-reactivity or interference between canine eNOS and analogues was observed.
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes	Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range	78.1 pg/ml - 5000 pg/ml
Sensitivity	62.5 pg/ml
Inter Assay	CV%<10%
Intra Assay	CV%<8%
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume	50-100ul
Applications	ELISA
Typical Data	ELISA: Canine eNOS ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme that in humans is encoded by the NOS3 gene located in the 7q35-7q36 region of chromosome 7. This enzyme is one of three isoforms that synthesize nitric oxide (NO), a small gaseous and lipophilic molecule that participates in several biological processes. The other isoforms include neuronal nitric oxide synthase (nNOS), which is constitutively expressed in specific neurons of the brain and inducible nitric oxide synthase (iNOS), whose expression is typically induced in inflammatory diseases. eNOS is primarily responsible for the generation of NO in the vascular endothelium, a monolayer of flat cells lining the interior surface of blood vessels, at the interface between circulating blood in the lumen and the remainder of the vessel wall. NO produced by eNOS in the vascular endothelium plays crucial roles in regulating vascular tone, cellular proliferation, leukocyte adhesion, and platelet aggregation. Therefore, a functional eNOS is essential for a healthy cardiovascular system.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Canine eNOS ELISA Kit

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