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Cathepsin D Antibody (monoclonal)

Catalog Number: ASA-B0311
Lead time: 1 week
$260.00

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

cathepsin D Monoclonal IgG Mouse Human IHC-P, WB Ascites

Immunogen

Human liver cathepsin D.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

CTSD

Protein

Cathepsin D

Uniprot ID

Human(P07339), Mouse(P18242), Rat(P24268)

Function

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Tissue Specificity

Expressed in the aorta extrcellular space (at protein level).

Sub-cellular localization

Lysosome. Melanosome. Secreted, extracellular space. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).

Sequence Similarities

Belongs to the peptidase A1 family.

Aliases

CLN10|CPSD|cathepsin D(lysosomal aspartyl protease)|ceroid-lipofuscinosis, neuronal 10

Application Details

Application Concentration* Species Validated Using**
Western blot 0.5-1μg/ml Human AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 1-2μg/ml Human AssaySolution's IHC/ICC Detection kit


AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

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IHC(P): Human Mammary Cancer Tissue
 
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.