Cyclin D1 Antibody

Catalog Number: ASA-B0532
Lead time: 1 week


Long Name

Antibody Type

Antibody Isotype


Species Reactivity

Validated Applications


cyclin D1 Polyclonal IgG Rabbit Human, Rat WB Immunogen affinity purified.


A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1(19-37aa DANLLNDRVLRAMLKAEET), different from the related mouse and rat sequences by two amino acids.





100 µg/vial


Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.


Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).


At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen




G1/S-specific cyclin-D1

Uniprot ID



Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

Tissue Specificity


Sub-cellular localization


Sequence Similarities

Belongs to the cyclin family. Cyclin D subfamily.


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Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat AssaySolution's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti- Cyclin D1 antibody, ASA-B0532, All Western blotting
All lanes: Anti-CCND1(ASA-B0532) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Predicted band size: 33KD
Observed band size: 33KD
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.