Human Actin-free Vitamin D Binding Protein (VDBP) ELISA Kit

Catalog Number: AYQ-E11319
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 2638
Gene Symbol VDBP
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human Actin-free Vitamin D Binding Protein (VDBP) . No significant cross-reactivity or interference between Human Actin-free Vitamin D Binding Protein (VDBP) and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range 0.4 -- 25 ng/ml
Sensitivity 0.32 ng/ml
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Actin-free Vitamin D Binding Protein (VDBP) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background Vitamin-D binding protein (VDBP, DBP, Gc-globulin) is a plasma protein produced in the liver. VDBP has several physiologically important functions such as transporting vitamin D and its metabolites to target cells, binding and sequestering globular actin and binding fatty acids to functioning in the immune system. VDBP is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of VDBP is approximately 50 kDa and the concentration of VDBP in human plasma is app. 400 μg/ml.