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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
2638 |
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Gene Symbol
|
VDBP |
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Species
|
Human |
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Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Actin-free Vitamin D Binding Protein (VDBP) . No significant cross-reactivity or interference between Human Actin-free Vitamin D Binding Protein (VDBP) and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
0.4 -- 25 ng/ml |
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Sensitivity
|
0.32 ng/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
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Typical Data
|
ELISA: Human Actin-free Vitamin D Binding Protein (VDBP) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Vitamin-D binding protein (VDBP, DBP, Gc-globulin) is a plasma protein produced in the liver. VDBP has
several physiologically important functions such as transporting vitamin D and its metabolites to target cells,
binding and sequestering globular actin and binding fatty acids to functioning in the immune system. VDBP
is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood
stream. The molecular mass of VDBP is approximately 50 kDa and the concentration of VDBP in human
plasma is app. 400 μg/ml. |
