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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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174 |
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Gene Symbol
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alpha-Fetoprotein |
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Synonym
|
AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human alpha-Fetoprotein. No significant cross-reactivity or interference between human alpha-Fetoprotein and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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78.1 pg/ml - 5000 pg/ml |
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Sensitivity
|
62.5 pg/ml |
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Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human alpha-Fetoprotein ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein[5][6] that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal development. It is thought to be the fetal analog of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. |
