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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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51378 |
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Gene Symbol
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AGP4 |
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Synonym
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AGP4; ANG3; ANG-3; ANG-4; ANG4angiopoietin-3; angiopoietin 4; Angiopoietin-3; Angiopoietin-4; ANGPT4; dJ824F16.2 (angiopoietin 4); MGC138181; MGC138183 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Angiopoietin-4. No significant cross-reactivity or interference between human Angiopoietin-4 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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0.313 ng/ml - 20 ng/ml |
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Sensitivity
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0.25 ng/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human Angiopoietin-4 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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Angiopoietin-4 is a protein that in humans is encoded by the ANGPT4 gene. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. |
