Human CCL28 ELISA Kit

Catalog Number: AYQ-E11214

Lead time: 3-4 business days

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Products specifications

Storage	Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID	56477
Gene Symbol	CCL28
Synonym	CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC
Species	Human
Specificity	This assay has high sensitivity and excellent specificity for detection of human CCL28. No significant cross-reactivity or interference between human CCL28 and analogues was observed.
Kit Components	Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes	Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range	62.5 pg/ml - 4000 pg/ml
Sensitivity	50 pg/ml
Inter Assay	CV%<10%
Intra Assay	CV%<8%
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume	50-100ul
Applications	ELISA
Typical Data	ELISA: Human CCL28 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background	Chemokine (C-C motif) ligand 28 (CCL28), also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans. Human CCL28 is encoded by an RNA transcript of 373 nucleotides and a gene with four exons. The gene codes for a 127-amino acid CCL28 protein with a 22-amino acid N-terminal signal peptide. It shares 76% nucleic acid identity and 83% amino acid similarity to the equivalent molecule in mouse. Sequence analysis has revealed CCL28 to be most similar to another CC chemokine called CCL27.

Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human CCL28 ELISA Kit

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