Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
|
Gene ID
|
939 |
Gene Symbol
|
CD27 |
Synonym
|
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of human CD27/TNFRSF7. No significant cross-reactivity or interference between human CD27/TNFRSF7 and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
125 pg/ml - 8000 pg/ml |
Sensitivity
|
100 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human CD27/TNFRSF7 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
CD27 is a member of the tumor necrosis factor receptor superfamily. It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |