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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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3561 |
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Gene Symbol
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IL-2 R gamma |
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Synonym
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CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Common gamma Chain/IL-2 R gamma. No significant cross-reactivity or interference between human Common gamma Chain/IL-2 R gamma and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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62.5 pg/ml - 4000 pg/ml |
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Sensitivity
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50 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human Common gamma Chain/IL-2 R gamma ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL-2RG, is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. The γc glycoprotein is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. This protein is located on the surface of immature blood-forming cells in bone marrow. One end of the protein resides outside the cell where it binds to cytokines and the other end of the protein resides in the interior of the cell where it transmits signals to the cell's nucleus. The common gamma chain partners with other proteins to direct blood-forming cells to form lymphocytes (a type of white blood cell). The receptor also directs the growth and maturation of lymphocyte subtypes: T cells, B cells, and natural killer cells. These cells kill viruses, make antibodies, and help regulate the entire immune system. |
