Human Complement component C4c ELISA Kit

Catalog Number: AYQ-E11356
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 720
Gene Symbol C4C
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human Complement component C4c . No significant cross-reactivity or interference between Human Complement component C4c and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human Complement component C4c ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background C4c is a component in the complement cascade and is a degradation product of C4b which is cleaved by C4b/C3b inactivator to yield C4d and C4c (soluble). C4c consists of a g-chain (30 kDa), a b-chain (75 kDa), and two a-domains denoted a3 (27 kDa) and a4 (14 kDa). C4c reacts with C1 and C2 to form C3 convertase of the classic activation pathway