Human EGF ELISA Kit

Catalog Number: AYQ-E11196
Lead time: 3-4 business days
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$0.00
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 1950
Gene Symbol EGF
Synonym beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Species Human
Background Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF was originally described as a secreted peptide found in the submaxillary glands of mice and in human urine. EGF has since been found in many human tissues including submandibular gland (submaxillary gland) and parotid gland. Initially, human EGF was known as urogastrone.
Typical Data ELISA: Human EGF ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Applications ELISA
Sample Volume 50-100ul
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Assay Type Sandwich ELISA
Intra Assay CV%<8%
Inter Assay CV%<10%
Sensitivity 3.125 pg/ml
Standard Curve Range 3.91 pg/ml - 250 pg/ml
Notes Please contact our Technical Services with any questions regarding species reactivity
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Specificity This assay has high sensitivity and excellent specificity for detection of human EGF. No significant cross-reactivity or interference between human EGF and analogues was observed.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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