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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
2161 |
|
Gene Symbol
|
F12 |
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Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Factor XII . No significant cross-reactivity or interference between Human Factor XII and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
40 -- 3840 ug/ml |
|
Sensitivity
|
32 ug/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
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Typical Data
|
ELISA: Human Factor XII ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Factor XII is a component in the coagulation cascade. It is a plasma glycoprotein that participates in the
initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein
is cleaved by factor XII to form kallikrein, which then cleaves factor XII to a-factor XIIa, which in turn
activates factor XI to factor XIa. |
