|
Standard Curve Range
|
7.8 - 500 pg/mL |
|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
|
Gene ID
|
2641 |
|
Gene Symbol
|
GCG |
|
Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Glucagon . No significant cross-reactivity or interference between Human Glucagon and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human Glucagon ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Glucagon is a peptide hormone of 29 amino-acid residues produced by the alpha cells of the pancreatic
islets. Its effect is to raise blood sugar as a stress hormone and counter-regulatory hormone for insulin. It
is produced by cell-type-specific processing of proglucagon, which in the alpha cells is split to form
glicentin-related pancreatic polypeptide, glucagon and major proglucagon fragment, while the same
precursor is split to form glicentin, oxyntomodulin (C-terminally extended glucagon-37), GLP-1 and GLP-2
in the L cells of the intestinal mucosa. |
