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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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3002 |
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Gene Symbol
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Granzyme B |
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Synonym
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C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Granzyme B. No significant cross-reactivity or interference between human Granzyme B and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
39.1 pg/ml - 2500 pg/ml |
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Sensitivity
|
31.25 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
|
ELISA |
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Typical Data
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ELISA: Human Granzyme B ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs), natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has also more recently found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The secondary functions of granzyme B are also numerous. Granzyme B has shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodelling. Elevated levels of granzyme B are also implicated in a number of autoimmune diseases, several skin diseases, and type 1 diabetes. |
