Human H-Ficolin ELISA Kit

Catalog Number: AYQ-E11350
Lead time: 3-4 business days
Products specifications
Storage Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID 2219
Gene Symbol FCN1
Species Human
Specificity This assay has high sensitivity and excellent specificity for detection of Human H-Ficolin . No significant cross-reactivity or interference between Human H-Ficolin and analogues was observed.
Kit Components Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes Please contact our Technical Services with any questions regarding species reactivity
Inter Assay CV%<10%
Intra Assay CV%<8%
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume 50-100ul
Applications ELISA
Typical Data ELISA: Human H-Ficolin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.