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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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10406 |
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Gene Symbol
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HE4 |
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Synonym
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dJ461P17.6; EDDM4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; HE4MGC57529; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WAP5epididymal protein 4; WFDC2 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human HE4/WFDC2. No significant cross-reactivity or interference between human HE4/WFDC2 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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62.5 pg/ml - 4000 pg/ml |
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Sensitivity
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50 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human HE4/WFDC2 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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WAP four-disulfide core domain protein 2 - also known as Human Epididymis Protein 4 (HE4) - is a protein that in humans is encoded by the WFDC2 gene. HE4 is a tumor marker of ovarian cancer, with 80% sensitivity at a cut-off of 150 pmol/L. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. |
