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Magnetic beads for protein/antibody and DNA purification, immunoprecipitation, CHIP assay
The Excel table for antibody affinity comparison
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Immunohistochemistry (IHC) - Premier
Immunohistochemistry (IHC)
Secondary Antibodies
Flow Cytometry
Conjugated Antibodies
Unconjugated Antibodies
Isotype Control Antibodies
Western Blot
Matched Antibody Pairs
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ELISA
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Human Hyaluronidase 1/HYAL1 ELISA Kit
Human Hyaluronidase 1/HYAL1 ELISA Kit
Catalog Number:
AYQ-E11273
Lead time:
3-4 business days
Please choose size:
*
96 T
96X2 T
96x5 T
$
0.00
Qty:
Products specifications
Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.
Gene ID
3373
Gene Symbol
HYAL1
Synonym
EC 3.2.1.35; FUS2; HYAL1; HYAL-1; Hyaluronidase 1; Hyaluronoglucosaminidase 1; hyaluronoglucosaminidase-1; LUCA1; luCa-1; LUCA1hyaluronidase-1; Lung carcinoma protein 1; MGC45987; NAT6; plasma hyaluronidase; tumor suppressor LUCA-1
Species
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human Hyaluronidase 1/HYAL1. No significant cross-reactivity or interference between human Hyaluronidase 1/HYAL1 and analogues was observed.
Kit Components
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual
Notes
Please contact our Technical Services with any questions regarding species reactivity
Standard Curve Range
12.5 pg/ml - 800 pg/ml
Sensitivity
10 pg/ml
Inter Assay
CV%<10%
Intra Assay
CV%<8%
Assay Type
Sandwich ELISA
Suitable Sample Type
serum, plasma, tissue homogenates, cell lysate, cell culture medium.
Sample Volume
50-100ul
Applications
ELISA
Typical Data
ELISA: Human Hyaluronidase 1/HYAL1 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Background
Hyaluronidase-1 is an enzyme that in humans is encoded by the HYAL1 gene. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Assay Solution's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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